【摘要】目的 综述国内外有关针刺戒毒的临床疗效和机理 的进展。方法 总结了耳针、体针、耳体结合举治疗方法在临床运用中的疗效，并简要阐述了针刺戒毒的可能机制。结果 为针刺戒毒的临床运用提供了可借鉴的资料。结论 针刺戒毒具有特效、安全、价廉、无明显副作用的特点。
[Abstract] Objective The author reviewed the advances of the clinical effect of acupuncture and its mechanism in stopping drug abuse in recent years. Method The author summarized the curative effect of clinical application of various needling methods， such as auricular acupuncture, body acupuncture and the combination of auricular and body acupuncture. The possibility of stopping drug abuse by acupuncture was expounded. Result To provide necessary data for the clinical application of acupuncture to stop drug abuse. Conclusion In stopping drug abuse, acupuncture therapy is highly effective，safe，cheap and free of side effect.
摘要 目的：从大鼠脾脏克隆信号分子guanine-nueleotide-releastng factor (GRF) pleckstrin homofogy (PH)结构域基因并迸行谷胱肽转移酶融合表达，为进一步寻找其新配基、研究其功 能打下基础。方法：采用一步法从大鼠新鲜脾组织中提取反转录合成及方法扩大增目的基因片段，并克隆到pUC19中。引物末端标记后，以双氧终止法测序。然后再克隆到表达载体PGEX-4T-1中作GST融合表达。结果:信号GRF的PH结构域基因完全正确，内部不含终止码。并在表达载体pGEX-4T-1中获得融合表达。结论：从组织细胞中克隆Ph结构域基因是可 行的方法，可在大肠杆菌中以GST融合蛋白的形式表达。
Abstract AIM: To screen for novel ligands of the PH domains and to investigate the functions of the PH domains in signal transduction. METHODS: Rat spleens were employed to clone the gene encoding PH domain of GRF. Total RNA molecules were isolated from fresh sheens and mRNAs were reversely transcriped into cDNAs. After amplication by PCR, the fragments of DNA were cloned into vector pUC19. The method of dideoxy-termination was employed to sequence with labelled primmers, and then the gene was cloned into vector pGEX-4T-1 ?o express in fusion with GST. RESULTS：The sequence of gene was correct and the gene was successfully expressed in pGEX-4T-1. CONCLUSION: The methods of cloning PH domains of signaling molecules are feasible and useful in study of signal trans-duction. The fragments of the gene can correctly be expressed in E, Coli as fusion with GST.